PREPARATION OF DIFFERENT SOLUTIONS

FROG’S RINGER SOLUTION
COMPOSITION:
NaCl: 0.65%
KCl: 0.014%
CaCl2 (Anhydrous): 0.012%
NaH2PO4 (Sodium Dihydrogen Phosphate: 0.001%
NaHCO3: 0.02%
Glucose: 0.2%
Water quantity sufficient to produce 100ml.

RINGER SOLUTION FOR MAMMALIANS
COMPOSITION:
NaCl: 0.9%
KCl: 0.042%
CaCl2 (Anhydrous): 0.024%
NaHCO3: 0.02%
Glucose: 0.2%
Water quantity sufficient to produce 100ml.

TYRODE’S SOLUTION (GUINEPIG ILEUM)
COMPOSITION:
NaCl: 0.8%
KCl: 0.02%
CaCl2 (Anhydrous): 0.02%
MgCl2: 0.01%
NaH2PO4 (Sodium Dihydrogen Phosphate): 0.005%
NaHCO3: 0.1%
Glucose: 0.1%
Water quantity sufficient to produce 100ml.

KREB’S SOLUTION
COMPOSITION:
NaCl: 0.68%
KCl: 0.035%
CaCl2 (Anhydrous): 0.028
MgSO4: 0.029%
KH2PO4: 0.016%
NaHCO3: 0.21%
Water quantity sufficient to produce 100ml.

NORMAL SALINE
COMPOSITION:
NaCl: 0.9%

HOW TO PREPARE
PROCEDURE:Except CaCl2 (anhydrous) very other ingredient is dissolved in water. CaCl2 is dissolved in another beaker and then it is transferred to the main solution by stirring.
NaH2PO4 maintains pH whereas Glucose provides energy.